Scientific Publications

Click the category headers to see the publilcation references.

Hepatitis A Virus (HAV)

J. Saldanha, A.Heath, N. Lelie, G. Pisani and M-Y. Yu. Report of the WHO Collaborative study to establish an International Standard for HAV RNA Nucleic Acid Amplification Technology (NAT) AssaysExpert Committee on Biological standardization, Geneva, 17-21 February 2003.

Hepatitis B Virus (HBV)

J.J.A.M. Weusten, A.A.J. van Drimmelen and P.N. Lelie. Mathematical modelling of the risk of HBV, HCV and HIV transmission by window phase donations not detected by NAT, Transfusion. 42: 537-48, 2002.

S. Sato, W. Ohhashi, H. Ihara et al. Comparison of the sensitivity of NAT using pooled donor samples for HBV and that of a serological HBsAg assay, Transfusion. 41: 1107-1113, 2001.

J. Saldanha, W.H. Gerhlich, P.N. Lelie, K.H. Heerman and A. Heath. An international Collaborative study to establish a WHO International Standard for Hepatitis B virus DNA Nucleic Amplification Techniques, Vox Sang. 80: 63-71, 2001.

K.H. Heerman, W.H. Gehrlich, M. Chudy, S. Schaefer, R. Thomsson and the Eurohep Pathobiology group. Quantitative detection of Hepatitis B virus DNA in two international reference preparations, J. Clin. Microbiol. 37: 68-73, 1999.

M.L. Collins, J.J. Zayetti, B. Detmer et al. Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays, Anal. Biochem. 226: 20-129, 1995.

W.G.V. Quint, R.A. Heijtink, J. Schirm, W.H. Gerlich and H.G.M. Niesters. Reliability of methods for hepatitis B virus DNA detection, J. Clin. Microbiol. 33: 25- 228, 1995.

P.N. Lelie, H.W. Reesink and C.J. Lucas. Inactivation of 12 viruses by heating steps applied during manufacture of a hepatitis B vaccine, J. Med. Virol. 23(3): 297-301, Nov 1987.

P.N. Lelie, H.W. Reesink, J. Niessen, B. Brotman and A.M. Prince. Inactivation of 10(15)chimpanzee infectious doses of hepatitis B virus during preparation of an heat-inactivated hepatitis B vaccine, J. Med. Virol. 23(3): 289-95, Nov 1987.

H.W. Reesink, E.E. Reerink-Brongers, H.G.J. Brummelhuis, P.N. Lelie, P.J. Dees, B.Th. Visser and W.G. van Aken. Overview of safety, immunogenicity and efficacy of heat-inactivated hepatitis B vaccine. Proceedings of 1984 International Symposium on Viral Hepatitis. 659; 1984.

Hepatitis C Virus (HCV)

L.Pembrey, M-L. Newell, P-A. Tovo, H. van Drimmelen, I. Quinti, G. Furlini, S. Galli, M.G. Meliconi, S. Burns, N. Hallam, A. Sonnerborg, G. Cilla, E. Serrano, P. Laccetti, G. Portella, S. Polywka, G. Icardi, B. Bruzzone, L. Balbo, A. Alfarano and the European Paediatric Hepatitis C virus Network. Inter-Laboratory Comparison of HCV-RNA Assay results: Implications for Multi-Centre Research, J. of Med. Virology. 69: 195-201, 2003.

J.M. Jongerius, M.C. Sjerps, H.T.M. Cuypers, A.A.J. van Drimmelen, C.L. van der Poel, H.W. Reesink, M.H.J. Molijn, G.A.H. Peeters, A.P.W. Peeters, F.E. Mollet and P.N. Lelie. Validation of NucliSens Extractor in combination with the Ampliscreen HCV and HIV assays for application in minipool screening, Transfusion. 42: 792-7, 2002.

P.N. Lelie, A.A.J. van Drimmelen, H.T.M. Cuypers, S.J. Best, S.L. Stramer, C. Hyland, J.-P. Allain, P. Moncharmont, C. Defer, C.M. Nubling, A. Glauser, M. da Silva Cardoso, J.-F. Viret, M. Lankinen, L. Grillner, U. Wirthmuller, J. Coste, V. Schottstedt, B. Masecar and E.M. Dax. Sensitivity of HCV-RNA and HIV-RNA blood screening assays, Transfusion. 42: 527-36, 2002.

J.J.A.M. Weusten, A.A.J. van Drimmelen and P.N. Lelie. Mathematical modeling of the risk of HBV, HCV and HIV transmission by window phase donations not detected by NAT, Transfusion. 42: 537-48, 2002.

P. Jorgensen, P. Neuwald. Standardized hepatitis C virus RNA panels for nucleic acid testing assays, J. of Clin. Virol. 20: 35-40; 2001.

H.T.M. Cuypers, M.H.J. Molijn, H.J. Bos, A.P.W. Peeters, C.L. van der Poel and P.N. Lelie. Validation of the NucliSens Extractor in combination with the hepatitis C virus Cobas Amplicor 2.0 assay in four laboratories in the Netherlands utilizing nucleic acid amplification technology for blood screening, Vox Sang. 81: 12-20, 2001.

J. Saldanha, A. Heath, N. Lelie, G. Pisani, M. Nubling, M. Yu. Calibration of HCV working reagents for NAT assays against the HCV international standard, Vox Sang. 78(4): 217-24, 2000.

J. Saldanha, P.N. Lelie, A. Heath and the WHO collaborative Study Group. Establishment of the First International Standard for Nucleic Acid Amplification Technology (NAT) assays for HCV-RNA, Vox Sang. 76: 149-158, 1999.

H.T.M. Cuypers, R. van Dijk, J.-F. Viret, V. Schottstedt, M.Lankinen, M. Cardoso and P.N. Lelie. European multi-center validation study of NucliSens extractor in combination with HCV Amplicor version 2.0 for HCV NAT screening of plasma pools, Biologicals. 27: 303-314, 1999.

M. Damen, P.Sillekens, M. Sjerps, R. Melsert, I. Frantzen, H.W. Reesink, P.N. Lelie, H.T.M. Cuypers. Stability of Hepatitis C virus RNA during specimen handling and storage prior to NASBA amplification, J. of Virol. Methods. 72 :175- 184, 1998.

N. Lelie, H.T.M. Cuypers, A.A.J. van Drimmelen and W.G.V. Quint. Quality assessment of Hepatitis C virus nucleic acid amplification methods, An international proficiency study, Transfusion. 25: 102-110, 1998.

M. Damen, H.T.M. Cuypers, H.L. Zaaijer et al. Characterisation of the EUROHEP HCV- RNA plasma standards in a collaborative study. Proceedings of the IX Triennial International Symposium on Viral Hepatitis and Liver Disease; Rome Italy. Turin: Edizioni Minerva Medica; 1997, 239-243, April 1996.

M. Damen, H.T.M Cuypers. H.L. Zaaijer, H.W. Reesink, W.P. Schaasberg, W.H. Gerlich, H.G.M. Niesters and P.N. Lelie. International collaborative study on the second EUROHEP HCV-RNA reference panel, J. Virol. Methods. 58:175-185, 1996.

J. Detmer, R. Lagier, J. Flynn, C. Zayati, J. Kolberg,M.L. Collins, M. Urdeaand R. Sanchez-Pescador. Accurate quantification of Hepatitis C virus (HCV) RNA from all HCV genotypes by using Branched-DNA technology, J. Clin. Microbiol. 34, 901-907, 1996.

H.L. Zaaijer, H.T.M. Cuypers, H.W. Reesink, I.N. Winkel, G. Gerken and P.N. Lelie. Reliability of polymerase chain reaction for detection of hepatitis C virus, Lancet. 341: 722-724, 1993.

H.T.M. Cuypers, D. Bresters, I.N. Winkel, H.W. Reesink, A.J. Weiner, M. Houghton, C.L. van der Poel and P.N. Lelie. Storage conditions of blood samples and primer selection affect the yield of cDNA polymerase chain reaction products of hepatitis C virus, J. Clin. Microbiol. 30(12):3220-4, Dec 1992.

Human Immunodeficiency Virus, Type 1 (HIV-1)

C. Davis, A. Heath, S.J. Best, I. Hewlett, P.N. Lelie, R. Schuurman and H. Holmes. Calibration of HIV-1 working reagents for nucleic acid amplification techniques against the1st international standard for HIV-1 RNA, J. Virol. Methods. 107(1):37-44, Jan 2003.

P.N. Lelie, A.A.J. van Drimmelen, H.T.M. Cuypers, S.J. Best, S.L. Stramer, C. Hyland, J.-P. Allain, P. Moncharmont, C. Defer, C.M. Nubling, A. Glauser, M. da Silva Cardoso, J.-F. Viret, M. Lankinen, L. Grillner, U. Wirthmuller, J. Coste, V. Schottstedt, B. Masecar and E.M. Dax. Sensitivity of HCV-RNA and HIV-RNA blood screening assays, Transfusion. 42: 527-36, 2002.

J.J.A.M. Weusten, A.A.J. van Drimmelen and P.N. Lelie. Mathematical modeling of the risk of HBV, HCV and HIV transmission by window phase donations not detected by NAT, Transfusion. 42: 537-48, 2002.

J.M. Jongerius, M.C. Sjerps, H.T.M. Cuypers, A.A.J. van Drimmelen, C.L. van der Poel, H.W. Reesink, M.H.J. Molijn, G.A.H. Peeters, A.P.W. Peeters, F.E. Mollet and P.N. Lelie. Validation of NucliSens Extractor in combination with the Ampliscreen HCV and HIV assays for application in minipool screening, Transfusion. 42: 792-7, 2002.

P. Neuwald, M. Funelas, J. DelCarmen, A. Raybold, P. Jorgenson. Results of the 2001 AcroMetrix HIV-1 resistance proficiency program, J. of Clin. Virol. 21: S55-S63, 2002.

H. Holmes, C. Davis, A. Heath, I. Hewlett and P.N. Lelie. An international collaborative study to establish the 1st International Standard for HIV-1-RNA for use in Nucleic Acid-Based Techniques, J. Virol. Methods 92: 141-150, 2001.

S.M. Bruisten, P. Oudshoorn, P. van Swieten, B. Boeser-Nunnink, P. van Aarle, S.P. Tondreau, H.T.M. Cuypers. Stability of HIV-1 RNA in blood during specimen handling and storage prior to amplification by NASBA-QT, J. of Virol. Methods. 67: 199-207, 1997.

B19 Parvovirus (Parvo)

J. Saldanha, P.N. Lelie, M.-Y. Yu and A. Heath. Establishment of the first World Health Organization International Standard for human parvovirus B19 DNA nucleic acid amplification techniques, Vox Sang. 82(1): 24-31, Jan 2002.

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